AF2320

antibody from R&D Systems

Targeting: IL36G IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF2320

Provider

R&D Systems

Product name

Human IL-36 gamma/IL-1F9 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human IL-36 gamma/IL-1F9 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, approximately 5% cross-reactivity with rhIL-37 is observed, and less than 1% cross-reactivity with rhIL-1 alpha , rhIL-1 beta , rhIL-36Ra, and rhIL-36 beta is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9NZH8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.

Scheiermann P, Bachmann M, Härdle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Mühl H
Scientific reports 2015 Feb 17;5:8521

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The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36γ.

Lian LH, Milora KA, Manupipatpong KK, Jensen LE
The Journal of investigative dermatology 2012 May;132(5):1346-53

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IL-36γ/IL-1F9, an innate T-bet target in myeloid cells.

Bachmann M, Scheiermann P, Härdle L, Pfeilschifter J, Mühl H
The Journal of biological chemistry 2012 Dec 7;287(50):41684-96

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human IL-36 gamma/IL-1F9 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 20 ng/mL Recombinant Human TNF-alpha (Catalog # 210-TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-36 gamma/IL-1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36 gamma/IL-1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.