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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-31844 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF417 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG.
- Concentration
- 0.81 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ZNF417 Polyclonal Antibody (Product # PA5-31844). Sample (30 µg of whole cell lysate). Lane A: IMR32 . 7.5% SDS PAGE. ZNF417 Polyclonal Antibody (Product # PA5-31844) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF417 Polyclonal Antibody detects ZNF417 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF417 protein stained by ZNF417 Polyclonal Antibody (Product # PA5-31844) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF417 Polyclonal Antibody detects ZNF417 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF417 protein stained by ZNF417 Polyclonal Antibody (Product # PA5-31844) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 xenograft, using ZNF417 (Product # PA5-31844) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
