16113-1-AP

antibody from Invitrogen Antibodies

Targeting: MTHFD1L DKFZP586G1517, FLJ21145, FTHFSDC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16113-1-AP

Provider

Invitrogen Antibodies

Product name

MTHFD1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GRMVVASDK SGQPVTADDL GVTGALTVLM KDAIKPNLMQ TLEGTPVFVH AGPFANIAHG NSSVLADKIA LKLVGEEGFV VTEAGFGADI GMEKFFNIKC RASGLVPNVV VLVATVRALK MHGGGPSVTA GVPLKKEYTE ENIQLVADGC CNLQKQIQIT QLFGVPVVVA LNVFKTDTRA EIDLVCELAK RAGAFDAVPC YHWSVGGKGS VDLARAVREA ASKRSRFQFL YDVQVPIVDK IRTIAQAVYG AKDIELSPEA QAKIDRYTQQ GFGNLPICMA KTHLSLSHQP DKKGVPRDFI LPISDVRASI GAGFIYPLVG TMSTMPGLPT RPCFYDIDLD TETEQVKGLF (427-775 aa encoded by BC017477)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 16113-1-AP) (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.