APR00329G

antibody from Leading Biology

Targeting: USP1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

APR00329G

Provider

Leading Biology

Product name

USP1 Antibody

Antibody type

Polyclonal

Description

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin­proteosomal mechanisms. USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNA­mediated translation synthesis (TLS) during DNA repair. USP1 interaction with UAF1, a WD40 repeat containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.