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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 14346-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RTRCLECES LTERREDFQD ISVPVQEDEL SKVEESSEIS PEPKTEMKTL RWAISQFASV ERIVGEDKYF CENCHHYTEA ERSLLFDKMP EVITIHLKCF AASGLEFDCY GGGLSKINTP LLTPLKLSLE EWSTKPTNDS YGLFAVVMHS GITISSGHYT ASVKVTDLNS LELDKGNFVV DQMCEIGKPE PLNEEEARGV VENYNDEEVS IRVGGNTQPS KVLNKKNVEA IGLLGGQKSK ADYELYNKAS NPDKVASTAF AENRNSETSD TTGTHESDRN KESSDQTGIN ISGFENKISY VVQSLKEYEG KWLLFDDSEV KVTEEKDFLN SLSPSTSPTS TPYLLFYKKL (437-785 aa encoded by BC050525)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of USP1 antibody (14346-1-AP, 1:1000) with si-Control and si-USP1 transfected HEK293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 14346-1-AP) (USP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-USP1 (IP:14346-1-AP, 3ug; Detection:14346-1-AP 1:300) with HEK-293 cells lysate 3200ug.
