Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C744604 - Provider product page
- Provider
- LSBio
- Product name
- CDG1C / ALG6 Antibody (N-Terminus) LS-C744604
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit anti-ALG6 antibody. Lane 1: HeLa Whole Cell lysate. Lane 2: MOLT4 Whole Cell lysate. Lane 3: K-562 Whole Cell lysate. Load: 10 µg per lane. Primary antibody: ALG6 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: TBS-Casein overnight at 4°C. Predicted/Observed size: 58 kDa ALG-6.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry with anti-ALG6 antibody showing ALG6 staining in the squamous epithelium of the uterine cervix as well as in inflammatory elements diffused in the stroma of human cervical cancer tissue at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human ALG6 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).