LS-C744605

antibody from LSBio

Targeting: ALG6

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C744605

Provider

LSBio

Product name

CDG1C / ALG6 Antibody (N-Terminus) LS-C744605

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of rabbit anti-ALG6 antibody. Lane 1: HeLa Whole Cell lysate. Lane 2: MOLT4 Whole Cell lysate. Lane 3: K-562 Whole Cell lysate. Load: 10 µg per lane. Primary antibody: ALG6 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: TBS-Casein overnight at 4°C. Predicted/Observed size: 58 kDa ALG-6.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry with anti-ALG6 antibody showing ALG6 staining in the squamous epithelium of the uterine cervix as well as in inflammatory elements diffused in the stroma of human cervical cancer tissue at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-human ALG6 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).