TA324916

antibody from OriGene

Targeting: EN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324916

Provider

OriGene

Product name

Rabbit polyclonal EN1 (Engrailed 1) Antibody (N-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal EN1 (Engrailed 1) Antibody (N-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

EN1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.394 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of lysates from NCI-H460,293,SH-SY5Y cell line (from left to right),using EN1 Antibody (N-term)(Cat. #TA324916). TA324916 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of U251 cell stained with EN1 Antibody (N-term)(Cat#TA324916).U251 cells were incubated with EN1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).EN1 immunoreactivity is localized to Nucleus significantly.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#TA324916), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

IHC