Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319364 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Fbp5A antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Fbp5A antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- FBXO5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.3 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein in a human HeLa whole cell lysate (lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Primary antibody was used at 1:500. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Affinity Purified anti-Fbp5a antibody shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.? Tissue was formalin-fixed and paraffin embedded. ?Brown color indicates presence of protein, blue color shows cell nuclei.? ?Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Validation comment
- IHC
