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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB3018 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3018, RRID:AB_1574714
- Product name
- EPHB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references EphB1 is underexpressed in poorly differentiated colorectal cancers.
Loss of expression of EphB1 protein in gastric carcinoma associated with invasion and metastasis.
Sheng Z, Wang J, Dong Y, Ma H, Zhou H, Sugimura H, Lu G, Zhou X
Pathobiology : journal of immunopathology, molecular and cellular biology 2008;75(5):274-80
Pathobiology : journal of immunopathology, molecular and cellular biology 2008;75(5):274-80
Loss of expression of EphB1 protein in gastric carcinoma associated with invasion and metastasis.
Wang JD, Dong YC, Sheng Z, Ma HH, Li GL, Wang XL, Lu GM, Sugimura H, Jin J, Zhou XJ
Oncology 2007;73(3-4):238-45
Oncology 2007;73(3-4):238-45
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHB1 polyclonal antibody (Cat # PAB3018) in mouse brain tissue. EPHB1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of EPHB1 in human gastric cancer tissues.(a) EPHB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment.(b) EPHB1 protein focally positively stained in well-differentiated gastric cancer cells.(c) EPHB1 protein is focally positive in poorly differentiated gastric cancer cells.(d) Loss of EPHB1 expression in gastric cancer cells. (Provided by Jian-dong Wang,Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine)
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
