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Western blot
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-111772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphB1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EphB1 was performed in mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes. Lane 1,2: EphB1 (extracellular) Antibody (Product # PA5-111772) at a dilution of 1:800. Lane 3,4: EphB1 (extracellular) Antibody preincubated with the negative control antigen.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of EphB1 in live intact rat PC12 cells. A) Extracellular staining of cells with EphB1 (extracellular) Antibody (Product # PA5-111772) (1:100), followed by goat anti-rabbit-AlexaFluor-594 (red). B) Live image of the cells. C) Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of EphB1 in live intact human THP-1 monocytic leukemia cell line. Black: Cells and goat anti-rabbit-AlexaFluor-488 secondary antibody. Green: Cells and EphB1 (extracellular) Antibody (Product # PA5-111772), (1:15) and goat-anti-rabbit-Alexa-488.
