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Learn17950-1-AP
antibody from Invitrogen Antibodies
Targeting: WLS
C1orf139, EVI, FLJ23091, GPR177, mig-14, MRP
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [12]
- Other assay [1]
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- Product number
- 17950-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR177 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LIAPGPTTA VSYMSVKCVD ARKNHHKTKW FVPWGPNHCD KIRDIEEAIP REIEANDIVF SVHIPLPHME MSPWFQFMLF ILQLDIAFKL NNQIRENAEV SMDVSLAYRD DAFAEWTEMA HERVPRKLKC TFTSPKTPEH EGRYYECDVL PFMEIGSVAH KFYLLNIRLP VNEKKKINVG IGEIKDIRLV GIHQNGGFTK VWFAMKT (34-239 aa encoded by BC110826)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.21 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of GPR177 antibody (17950-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPR177 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 17950-1-AP (GPR177 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 17950-1-AP (GPR177 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 17950-1-AP (GPR177 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary using 17950-1-AP (GPR177 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GPR177 (IP:17950-1-AP, 3ug; Detection:17950-1-AP 1:500) with mouse liver tissue lysate 12500ug.
