12653-1-AP

antibody from Invitrogen Antibodies

Targeting: ARMC8 DKFZP434A043, GID5, HSPC056, VID28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12653-1-AP

Provider

Invitrogen Antibodies

Product name

ARMC8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEVTASSRH YVDRLFDPDP QKVLQGVIDM KNAVIGNNKQ KANLIVLGAV PRLLYLLQQE TSSTELKTEC AVVLGSLAMG TENNVKSLLD CHIIPALLQG LLSPDLKFIE ACLRCLRTIF TSPVTPEELL YTDATVIPHL MALLSRSRYT QEYICQIFSH CCKGPDHQTI LFNHGAVQNI AHLLTSLSYK VRMQALKCFS VLAFENPQVS MTLVNVLVDG ELLPQIFVKM LQRDKPIEMQ LTSAKCLTYM CRAGAIRTDD NCIVLKTLPC LVRMCSKERL LEERVEGAET LAYLIEPDVE LQRIASITDH LIAMLADYFK YPSSVSAITD IKRLDHDLKH AHELRQAAFK L (1-350 aa encoded by BC013424)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12653-1-AP (ARMC8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 12653-1-AP (ARMC8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12653-1-AP (ARMC8 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).