Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47745 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLSTN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Calsyntenin-1 and rhCalsyntenin-3 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Calsyntenin-2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-human Calsyntenin-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47745) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained using the Anti-Sheep HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to neurons.