PA5-56987

antibody from Invitrogen Antibodies

Targeting: CCDC115 ccp1, FLJ30131, MGC12981

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56987

Provider

Invitrogen Antibodies

Product name

CCDC115 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PREAGLRRRK GPTKTPEPES SEAPQDPLNW FGILVPHSLR QAQASFRDGL QLAADIASLQ NRIDWGRSQL RGLQEKLKQL EP

Concentration

0.4 mg/mL

References

Submitted references

Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication.

Vuono EA, Ramirez-Medina E, Berggren K, Rai A, Pruitt S, Silva E, Velazquez-Salinas L, Gladue DP, Borca MV
Viruses 2020 Mar 31;12(4)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CCDC115 in human colon tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a CCDC115 Polyclonal Antibody (Product # PA5-56987).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Interaction between CSFV E2 and CCDC115 in CSFV-infected cells demonstrated by proximity ligation assay (PLA). Images were visualized at 40X magnification. SK6 cells ( A ) or primary swine macrophages ( B ) that were either mock infected (Mock) or infected for 24 h with CSFV parental BICv or recombinant E2DeltaCCDC115v (MOI = 10) as described in Materials and Methods. Panel B (on the right), quantification of the decrease in the E2-CCDC115 interaction in swine macrophages infected with either BICv or E2DeltaCCDC115v, error bars represent the average and standard deviation from from the counting of three independent counts of 100 cells. *** p -value < 0.0008.