Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-87499 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87499, RRID:AB_11034277
- Product name
- Rabbit Polyclonal GABA-AR alpha 3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse GABA A R alpha 3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.
Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA A R alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA A R alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GABA A R alpha 3 Antibody [NBP1-87499] - Analysis in control (vector only transfected HEK293T lysate) and GABRA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA A R alpha 3 Antibody [NBP1-87499] - Staining of mouse ventral pallidum shows strong labeling of synaptic fields.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA A R alpha 3 Antibody [NBP1-87499] - Staining of mouse olfactory bulb shows labelling of both neurons and synapses.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA A R alpha 3 Antibody [NBP1-87499] - Staining of mouse insular cortex shows strong immunoreactivity in synapses.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GABA A R alpha 3 Antibody [NBP1-87499] - Staining of mouse thalamus shows strong positivity in the paraventricular thalamic nucleus.
