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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- TA500635 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500635, RRID:AB_2299466
- Product name
- Anti-PDE10A mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)
- Antibody type
- Monoclonal
- Description
- Anti-PDE10A mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDE10A
- Isotype
- IgG
- Antibody clone number
- OTI1B2
- Vial size
- 100 µl
- Concentration
- 0.6 mg/ml
Submitted references Cyclic nucleotide phosphodiesterases in human spermatozoa and seminal fluid: Presence of an active PDE10A in human spermatozoa.
Maréchal L, Guillemette C, Goupil S, Blondin P, Leclerc P, Richard FJ
Biochimica et biophysica acta. General subjects 2017 Feb;1861(2):147-156
Biochimica et biophysica acta. General subjects 2017 Feb;1861(2):147-156
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE10A monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A .
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PDE10A monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500635, Dilution 1:50)
- Validation comment
- IHC
