13753-1-AP

antibody from Invitrogen Antibodies

Targeting: RNASET2 bA514O12.3, FLJ10907, RNASE6PL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13753-1-AP

Provider

Invitrogen Antibodies

Product name

RNase T2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DKRLRDNHE WKKLIMVQHW PETVCEKIQN DCRDPPDYWT IHGLWPDKSE GCNRSWPFNL EEIKDLLPEM RAYWPDVIHS FPNRSRFWKH EWEKHGTCAA QVDALNSQKK YFGRSLELYR ELDLNSVLLK LGIKPSINYY QVADFKDALA RVYGVIPKIQ CLPPSQDEEV QTIGQIELCL TKQDQQLQNC TEPGEQPSPK QEVWLANGAA ESRGLRVCED GPVFYPPPKK TKH (25-256 aa encoded by BC039713)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13753-1-AP (RNase T2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13753-1-AP (RNase T2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 13753-1-AP (RNase T2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 13753-1-AP (RNase T2 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RNase T2 (IP:13753-1-AP, 4ug; Detection:13753-1-AP 1:300) with BxPC-3 cells lysate 4000ug.