PAB22871

antibody from Abnova Corporation

Targeting: DHX36 DDX36, KIAA1488, MLEL1, RHAU

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22871

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22871, RRID:AB_10961701

Product name

DHX36 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DHX36.

Antigen sequence

ERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHG
YGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIR
NRSYIDRDSEYLLQENEPDGT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with DHX36 polyclonal antibody (Cat # PAB22871) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with DHX36 polyclonal antibody (Cat # PAB22871) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with DHX36 polyclonal antibody (Cat # PAB22871) shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)