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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00055777-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055777-M01, RRID:AB_426022
- Product name
- MBD5 monoclonal antibody (M01), clone 4A12-1B6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MBD5.
- Antigen sequence
MFPPTANMLLPTGEGQSGRAALRDKLMSQQKDALR
KRKQPPTTVLSLLRQSQMDSSAVPKPGPDLLRKQG
QGSFPISSMSQLLQSMSCQSSHLSSNSTPGCGASN
TALPCSANQLHFTDPSMNSSVLQNIPLRGEAVHCH
NANTNFVHSNSPVPNHHLAGLINQIQASGNCGMLS
QSGMALGNSLHPNPPQSRISTSSTPVIPNSIVSSY
NQTSSEAGMVLLEKSTQRY- Isotype
- IgG
- Antibody clone number
- 4A12-1B6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MBD5 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
