H00009197-M07

antibody from Abnova Corporation

Targeting: SLC33A1 ACATN, AT-1, AT1, SPG42

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009197-M07

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009197-M07, RRID:AB_566190

Product name

SLC33A1 monoclonal antibody (M07), clone 3A4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SLC33A1.

Antigen sequence

MSPTISHKDSSRQRRPGNFSHSLDMKSGPLPPGGW
DDSHLDSAGREGDREALLGDTGTGDFLKAPQSFR


Isotype

IgG

Antibody clone number

3A4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

SLC33A1/AT-1 protein regulates the induction of autophagy downstream of IRE1/XBP1 pathway.

Pehar M, Jonas MC, Hare TM, Puglielli L
The Journal of biological chemistry 2012 Aug 24;287(35):29921-30

---

AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.

Jonas MC, Pehar M, Puglielli L
Journal of cell science 2010 Oct 1;123(Pt 19):3378-88

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SLC33A1 monoclonal antibody (M07), clone 3A4 Western Blot analysis of SLC33A1 expression in PC-12 ( Cat # L012V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SLC33A1 expression in transfected 293T cell line by SLC33A1 monoclonal antibody (M07), clone 3A4.Lane 1: SLC33A1 transfected lysate(60.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SLC33A1 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol