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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00011146-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00011146-M01, RRID:AB_425886
- Product name
- GLMN monoclonal antibody (M01), clone 1C12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant GLMN.
- Antigen sequence
MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQR
CIEEGHTDQLLEIIQNEKNKVIIKNMGWNLVGPVV
RCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLG
LLELIEEPSGKQISQSILLLLQPLQTVIQKLHNKA
YSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQ
CCKALIEFTKPFVEEVIDNKENSLENEKLKDELLK
FCFKSLKCPLLTAQFFEQSEEGGNDPFRYFASEII
GFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENK
QLADSMASLAYLVFVQGIHIDQLPMVLSPLYLLQF
NMGHIEVFLQRTEESVISKGLELLENSLLRIEDNS
LLYQYLEIKSFLTVPQGLVKVMTLCPIETLRKKSL
AMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI
IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLF
LPEGAETDLLQNSDRIMASLNLLRYLVIKDNENDN
QTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIK
NSQEAQKSKDLCSITVSGEEIPNMPPEMQLKVLHS
ALFTFDLIESVLARVEELIEIKTKSTSEENIGIK- Isotype
- IgG
- Antibody clone number
- 1C12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GLMN monoclonal antibody (M01), clone 1C12. Western Blot analysis of GLMN expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody (M01), clone 1C12.Lane 1: GLMN transfected lysate(68.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GLMN is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of GLMN transfected lysate using anti-GLMN monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLMN MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
