MAB6618

antibody from Novus Biologicals

Targeting: RND3 ARHE, Rho8, RhoE

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6618

Provider

Novus Biologicals

Product name

Mouse Monoclonal RND3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human RND3 in direct ELISAs and Western blots.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TGF-β1 stimulates human Tenon's capsule fibroblast proliferation by miR-200b and its targeting of p27/kip1 and RND3.

Tong J, Fu Y, Xu X, Fan S, Sun H, Liang Y, Xu K, Yuan Z, Ge Y
Investigative ophthalmology & visual science 2014 Apr 28;55(4):2747-56

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human RND3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RND3 (RD1 Peptide) Monoclonal Antibody (Catalog # MAB6618) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RND3 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.