ALX-210-365-C050

antibody from Enzo Life Sciences

Targeting: PTX3 TNFAIP5, TSG-14

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ALX-210-365-C050

Provider

Enzo Life Sciences

Product name

PTX3 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant full length protein

Description

Affinity purified.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Vial size

50 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Cigarette smoke induces PTX3 expression in pulmonary veins of mice in an IL-1 dependent manner.

Pauwels NS, Bracke KR, Maes T, Van Pottelberge GR, Garlanda C, Mantovani A, Joos GF, Brusselle GG
Respiratory research 2010 Oct 4;11:134

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Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease.

Boehme M, Kaehne F, Kuehne A, Bernhardt W, Schröder M, Pommer W, Fischer C, Becker H, Müller C, Schindler R
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2007 Aug;22(8):2224-9

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Elevated maternal levels of the long pentraxin 3 (PTX3) in preeclampsia and intrauterine growth restriction.

Cetin I, Cozzi V, Pasqualini F, Nebuloni M, Garlanda C, Vago L, Pardi G, Mantovani A
American journal of obstetrics and gynecology 2006 May;194(5):1347-53

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A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel.

Duzhyy D, Harvey M, Sokolowski B
The Journal of biological chemistry 2005 Apr 15;280(15):15165-72

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The long pentraxin PTX3 is synthesized in IgA glomerulonephritis and activates mesangial cells.

Bussolati B, Peri G, Salvidio G, Verzola D, Mantovani A, Camussi G
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1466-72

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PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans.

Peri G, Introna M, Corradi D, Iacuitti G, Signorini S, Avanzini F, Pizzetti F, Maggioni AP, Moccetti T, Metra M, Cas LD, Ghezzi P, Sipe JD, Re G, Olivetti G, Mantovani A, Latini R
Circulation 2000 Aug 8;102(6):636-41

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Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan.

Vouret-Craviari V, Matteucci C, Peri G, Poli G, Introna M, Mantovani A
Infection and immunity 1997 Apr;65(4):1345-50

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Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes.

Alles VV, Bottazzi B, Peri G, Golay J, Introna M, Mantovani A
Blood 1994 Nov 15;84(10):3483-93

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Pulmonary PTX3 expression by immunohistochemistry (IHC) using PTX3, pAb (Prod. No. ALX-210-365). Method: Photomicrograph of PTX3 stained lung tissue of Cigarette Smoke (CS)-exposed WT mice. Mice were exposed to CS for 4 weeks (subacute).(V: vein, A: artery, AW: airway. Magnification 200_). In courtesy of Guy Brusselle.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Lysates of normal lung fibroblasts (WI38) and of human fibrosarcoma (8387) were probed with the PTX3, pAb, (Prod. No. ALX-210-365), over night at +4 ¡C at a concentration of 500ng/ml in 5% dry milk in PBS+/+ containing 0.05% Tween 20. The signal was revealed with an HRP-conjugated anti-rat IgG secondary antibody in 5% dry milk PBS+/+ containing 0.05% Tween 20 after incubation for 1 hour at room temperature and IMMOBILON Western Chemiluminescent substrate from Millipore.