ALX-804-803-C100

antibody from Enzo Life Sciences

Targeting: NLRP1 CARD7, CLR17.1, DEFCAP, DKFZp586O1822, KIAA0926, NAC, NALP1, SLEV1, VAMAS1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ALX-804-803-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-803-C100, RRID:AB_2051967

Product name

NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

Nalpy1-4

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.

References

Submitted references

Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response.

Kummer JA, Broekhuizen R, Everett H, Agostini L, Kuijk L, Martinon F, van Bruggen R, Tschopp J
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2007 May;55(5):443-52

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of the time course of assembly of the inflammasome usingÊMAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages. Method: Assembly of the inflammasome was induced by shifting the temperature to 30¡C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated usingÊPAbÊtoÊAsc (AL177) (Prod. No. ALX-210-905) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5'000 dilution for ECL detection. For more information about the \time course assembly of inflammasome\ method see F. Martinon; Mol. Cell. 10, 417 (2002).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4). Method: 293T cells transfected with full length human NLRP1/NALP1Êwere fixed with 4% paraformaldehyde and stained with 1µg/mlÊMAb to NLRP1/NALP1 (Nalpy1-4)Ê(Prod. No. ALX-804-803) and then Alexa 488 (1:300 dilution).