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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- 600-401-871 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-871, RRID:AB_2179985
- Product name
- Anti-Mouse Rif1 (RABBIT) Antibody - 600-401-871
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Epigenetic Regulation of the Blimp-1 Gene (Prdm1) in B Cells Involves Bach2 and Histone Deacetylase 3.
Tanaka H, Muto A, Shima H, Katoh Y, Sax N, Tajima S, Brydun A, Ikura T, Yoshizawa N, Masai H, Hoshikawa Y, Noda T, Nio M, Ochiai K, Igarashi K
The Journal of biological chemistry 2016 Mar 18;291(12):6316-30
The Journal of biological chemistry 2016 Mar 18;291(12):6316-30
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
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- Experimental details
- Western blot using Rockland's Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0?ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
