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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX131889 - Provider product page
- Provider
- GeneTex
- Product name
- RIF1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Rif1 antibody detects Rif1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rif1 antibody (GTX131889) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rif1 antibody detects Rif1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rif1 protein stained by Rif1 antibody (GTX131889) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Rif1 protein from HeLa whole cell extracts using 5 £gg of Rif1 antibody (GTX131889).Western blot analysis was performed using Rif1 antibody (GTX131889).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Rif1 antibody (GTX131889) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Rif1 antibody (GTX131889) diluted at 1:500.
