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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- GTX85728 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX85728, RRID:AB_10732196
- Product name
- RIF1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Kidney (formalin-fixed, paraffin-embedded) stained with RIF1 antibody (GTX85728) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human Testis (formalin-fixed, paraffin-embedded) stained with RIF1 antibody (GTX85728) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
