27580-1-AP

antibody from Invitrogen Antibodies

Targeting: ACTRT3 ARPM1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

27580-1-AP

Provider

Invitrogen Antibodies

Product name

ARPM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MIKAGVAGC REPQFIYPNI IGRAKGQSRA AQGGLELCVG DQAQDWRSSL FISYPVERGL ITSCEDMEIM WKHIYDYNLK LKPCDGPVLI TEPALNPLAN RQQITEMFFE HLGVPAFYMS IQAVLALFAA GFTTGLVLNS GAGVTQSVPI FEGYCLPHGV QQLDLAGLDL TNYLMVLMKN HGIMLLSASD RKIVEDIKES FCYVAMNYEE EMAKKPDCL (16-233 aa encoded by BC007289)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 27580-1-AP (ARPM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed U2OS cells using 27580-1-AP (ARPM1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed U2OS cells using 27580-1-AP (ARPM1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 27580-1-AP (ARPM1 antibody) at dilution of 1:200 (under 40x lens).