12596-1-AP

antibody from Invitrogen Antibodies

Targeting: OLFM3 NOE3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12596-1-AP

Provider

Invitrogen Antibodies

Product name

OLFM3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NLSAVLTGI QEEIGAYDYE ELHQRVLSLE TRLRDCMKKL TCGKLMKITG PVTVKTSGTR FGAWMTDPLA SEKNNRVWYM DSYTNNKIVR EYKSIADFVS GAESRTYNLP FKWAGTNHVV YNGSLYFNKY QSNIIIKYSF DMGRVLAQRS LEYAGFHNVY PYTWGGFSDI DLMADEIGLW AVYATNQNAG NIVISQLNQD TLEVMKSWST GYPKRSAGES FMICGTLYVT NSHLTGAKVY YSYSTKTSTY EYTDIPFHNQ YFHISMLDYN ARDRALCAWN NGHQVLFNVT LFHIIKTEDD T (179-478 aa encoded by BC022531)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP ( OLFM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A2780 cells were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 12596-1-AP (OLFM3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using OLFM3 antibody 12596-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12596-1-AP ( OLFM3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12596-1-AP ( OLFM3 Antibody) at dilution of 1:50 (under 40x lens).