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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-46657 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-46657, RRID:AB_626668
- Product name
- Anti-AMY1A
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Evidence for epithelial-mesenchymal transition in adult human pancreatic exocrine cells.
A small molecule that directs differentiation of human ESCs into the pancreatic lineage
Fanjul M, Gmyr V, Sengenès C, Ratovo G, Dufresne M, Lefebvre B, Kerr-Conte J, Hollande E
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Sep;58(9):807-23
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Sep;58(9):807-23
A small molecule that directs differentiation of human ESCs into the pancreatic lineage
Shuibing Chen, Malgorzata Borowiak, Julia L Fox, René Maehr, Kenji Osafune, Lance Davidow, Kelvin Lam, Lee F Peng, Stuart L Schreiber, Lee L Rubin, Douglas Melton
Nature Chemical Biology 2009 Mar;5(4):258-265
Nature Chemical Biology 2009 Mar;5(4):258-265
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 58
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
