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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09324PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09324PU-N, RRID:AB_2035445
- Product name
- anti ECT2 pThr790
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.31 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and nonphosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000.
