653902

antibody from BioLegend

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

653902

Provider

BioLegend

Proper citation

BioLegend Cat#653902, RRID:AB_2561778

Product name

Purified anti-ASC (TMS-1)

Antibody type

Monoclonal

Antigen

Recombinant human ASC (aa 113-195).

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

HASC-71

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

4° C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HL-60 whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ASC antibody (clone HASC-71). Proteins were visualized using anti-mouse IgG secondary antibody conjugated to HRP and chemiluminescence detection.

Conjugate

Unconjugated