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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6098 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6098, RRID:AB_1579441
- Product name
- PYCARD polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of PYCARD.
- Antigen sequence
C-RESQSYLVEDLERS- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway.
Ohtsuka T, Ryu H, Minamishima YA, Macip S, Sagara J, Nakayama KI, Aaronson SA, Lee SW
Nature cell biology 2004 Feb;6(2):121-8
Nature cell biology 2004 Feb;6(2):121-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PYCARD polyclonal antibody (Cat # PAB6098) staining (1 ug/mL) of U-937 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing PYCARD and probed with PYCARD polyclonal antibody (Cat # PAB6098) (mock transfection in first lane).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PYCARD polyclonal antibody (Cat # PAB6098)(2.5 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
