ADI-905-173-100

antibody from Enzo Life Sciences

Targeting: PYCARD ASC, CARD5, TMS-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ADI-905-173-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-173-100, RRID:AB_2038839

Product name

ASC polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide affinity purified.

Reactivity

Human

Host

Rabbit

Vial size

100 μg

Storage

-20°C

References

Submitted references

Lysosomal storage and impaired autophagy lead to inflammasome activation in Gaucher macrophages.

Aflaki E, Moaven N, Borger DK, Lopez G, Westbroek W, Chae JJ, Marugan J, Patnaik S, Maniwang E, Gonzalez AN, Sidransky E
Aging cell 2016 Feb;15(1):77-88

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Oligomerized CARD16 promotes caspase-1 assembly and IL-1β processing.

Karasawa T, Kawashima A, Usui F, Kimura H, Shirasuna K, Inoue Y, Komada T, Kobayashi M, Mizushina Y, Sagara J, Takahashi M
FEBS open bio 2015;5:348-56

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Genipin suppresses NLRP3 inflammasome activation through uncoupling protein-2.

Rajanbabu V, Galam L, Fukumoto J, Enciso J, Tadikonda P, Lane TN, Bandyopadhyay S, Parthasarathy PT, Cho Y, Cho SH, Lee YC, Lockey RF, Kolliputi N
Cellular immunology 2015 Sep;297(1):40-5

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The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.

Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD
Nature medicine 2015 Mar;21(3):263-9

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A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.

Coll RC, Robertson AA, Chae JJ, Higgins SC, Muñoz-Planillo R, Inserra MC, Vetter I, Dungan LS, Monks BG, Stutz A, Croker DE, Butler MS, Haneklaus M, Sutton CE, Núñez G, Latz E, Kastner DL, Mills KH, Masters SL, Schroder K, Cooper MA, O'Neill LA
Nature medicine 2015 Mar;21(3):248-55

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Staphylococcus aureus Leukocidin A/B (LukAB) Kills Human Monocytes via Host NLRP3 and ASC when Extracellular, but Not Intracellular.

Melehani JH, James DB, DuMont AL, Torres VJ, Duncan JA
PLoS pathogens 2015 Jun;11(6):e1004970

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Role of NLRP3 Inflammasomes for Rhabdomyolysis-induced Acute Kidney Injury.

Komada T, Usui F, Kawashima A, Kimura H, Karasawa T, Inoue Y, Kobayashi M, Mizushina Y, Kasahara T, Taniguchi S, Muto S, Nagata D, Takahashi M
Scientific reports 2015 Jun 5;5:10901

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Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome.

Wei Q, Guo P, Mu K, Zhang Y, Zhao W, Huai W, Qiu Y, Li T, Ma X, Liu Y, Chen X, Han L
Laboratory investigation; a journal of technical methods and pathology 2015 Jul;95(7):804-16

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Anti-inflammatory effect of emodin via attenuation of NLRP3 inflammasome activation.

Han JW, Shim DW, Shin WY, Heo KH, Kwak SB, Sim EJ, Jeong JH, Kang TB, Lee KH
International journal of molecular sciences 2015 Apr 10;16(4):8102-9

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Multiple binding sites on the pyrin domain of ASC protein allow self-association and interaction with NLRP3 protein.

Vajjhala PR, Mirams RE, Hill JM
The Journal of biological chemistry 2012 Dec 7;287(50):41732-43

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Varicella-zoster virus infection triggers formation of an interleukin-1β (IL-1β)-processing inflammasome complex.

Nour AM, Reichelt M, Ku CC, Ho MY, Heineman TC, Arvin AM
The Journal of biological chemistry 2011 May 20;286(20):17921-33

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide (2287P) with ASC antibody at 1µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunocytochemistry analysis of ASC in HL60 cells with ASC antibody at 5µg/ml.