Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Other assay [2]
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Validation data
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- Product number
- PA5-99458 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Mesoporous Polydopamine Loaded Pirfenidone Target to Fibroblast Activation Protein for Pulmonary Fibrosis Therapy.
Fang Q, Liu S, Cui J, Zhao R, Han Q, Hou P, Li Y, Lv J, Zhang X, Luo Q, Wang X
Frontiers in bioengineering and biotechnology 2022;10:920766
Frontiers in bioengineering and biotechnology 2022;10:920766
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAP in LOVO cell extract. Samples were incubated with FAP polyclonal antibody (Product # PA5-99458).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAP in HeLa cells. Samples were incubated with FAP polyclonal antibody (Product # PA5-99458) using a dilution of 1:100 (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAP in HeLa cells. Samples were incubated with FAP polyclonal antibody (Product # PA5-99458) using a dilution of 1:100 (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAP in LOVO cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with FAP polyclonal antibody (Product # PA5-99458) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 7 (A) IHC staining for FN, FAP, and alpha-SMA among different treatment groups. (B-D) AOD of IHC staining for FN, FAP, and alpha-SMA among different treatment groups.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 8 (A,B) WB results of FN and FAP among different treatment groups (* p < 0.05).