LS-C312938

antibody from LSBio

Targeting: HSPE1 CPN10, EPF, GroES, HSP10

Western blot

Antibody data

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Validation data

Product number: LS-C312938 - Provider product page
Provider: LSBio
Product name: HSPE1 / HSP10 / Chaperonin 10 Antibody (aa81-102) LS-C312938
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human, Rat, Bovine, Goat, Horse, Porcine, Rabbit, Sheep, Simian
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

HSPE1 protein structure - LS-C312938 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: IHC analysis of Cpn10 using anti-Cpn10 antibody. Cpn10 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Cpn10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Human Intestinal Cancer Tissue.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: HSPE1 / HSP10 / Chaperonin 10 antibody. IHC(P): Mouse Intestine Tissue.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: IHC analysis of Cpn10 using anti-Cpn10 antibody. Cpn10 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Cpn10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.