AF2584

antibody from R&D Systems

Targeting: HSPE1 CPN10, EPF, GroES, HSP10

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2584

Provider

R&D Systems

Product name

Mouse HSP10/EPF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse HSP10/EPF in Western blots.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q64433


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse HSP10/EPF by Western Blot. Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse HSP10/EPF by Simple Western^TM. Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.