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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-20745 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AP2M1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human kidney tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
Brigidi GS, Santyr B, Shimell J, Jovellar B, Bamji SX
Nature communications 2015 Sep 3;6:8200
Nature communications 2015 Sep 3;6:8200
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human kidney tissue lysate using a AP2M1 polyclonal antibody (Product # PA5-20745) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 Polyclonal Antibody (Product # PA5-20745) at (A) 1 and (B) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse kidney cells using a AP2M1 polyclonal antibody (Product # PA5-20745) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of AP2M1 in Mouse Kidney cells with AP2M1 Polyclonal Antibody (Product # PA5-20745) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of AP2M1 in mouse kidney tissue with AP2M1 Polyclonal Antibody (Product # PA5-20745) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 PSD-95 enhances the binding and tyrosine phosphorylation of DHHC5 by Fyn kinase. ( a - d ) Fourteen DIV hippocampal neurons were stimulated, lysed at the indicated time points, immunoprecipitated with anti- ( a ) DHHC5 or ( c ) Fyn and blots probed with the indicated antibodies. ( a , b ) DHHC5 tyrosine phosphorylation (phY; P =0.007, F 6,14 =7.749) and DHHC5/Fyn interactions ( P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Phosphorylation of DHHC5 regulates its association with endocytic proteins and its subcellular localization. ( a ) Schematic depiction of DHHC5 constructs N-terminally tagged with GFP or HA (not shown here) and illustrating the approximate localization of transmembrane domains (grey boxes), the DHHC motif, a putative Fyn-binding site (dashed line; RLLPTGP), a putative AP2mu-binding site (dashed line; YDNL) and the PDZ-binding motif required for binding PSD-95 (solid line; EISV). ( b - e ) HEK293T cells were transfected with the indicated HA-DHHC5 and Fyn constructs for 36 h, lysates immunoprecipitated with an HA antibody and blots probed with the indicated antibodies. ( b , c ) Fyn binding of the DHHC5 P520,3A mutant is reduced, but not for for the Y533E mutant ( P =0.0153, F 2,6 =9.07). ( b , d ) Fyn-mediated tyrosine phosphorylation is attenuated in DHHC5 P520,3A and Y533E mutants ( P
