14748-1-AP

antibody from Invitrogen Antibodies

Targeting: PSMD2 MGC14274, P97, Rpn1, S2, TRAP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14748-1-AP

Provider

Invitrogen Antibodies

Product name

PSMD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GLGLNHLGK GEAIEAILAA LEVVSEPFRS FANTLVDVCA YAGSGNVLKV QQLLHICSEH FDSKEKEEDK DKKEKKDKDK KEAPADMGAH QGVAVLGIAL IAMGEEIGAE MALRTFGHLL RYGEPTLRRA VPLALALISV SNPRLNILDT LSKFSHDADP EVSYNSIFAM GMVGSGTNNA RLAAMLRQLA QYHAKDPNNL FMVRLAQGLT HLGKGTLTLC PYHSDRQLMS QVAVAGLLTV LVSFLDVRNI ILGKSHYVLY GLVAAMQPRM LVTFDEELRP LPVSVRVGQA VDVVGQAGKP KTITGFQTHT TPVLLAHGER AELATEEFLP VTPILEGFVI LRKNPNYDL (561-908 aa encoded by BC002368)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP ( PSMD2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14748-1-AP (PSMD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 14748-1-AP (PSMD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 14748-1-AP) (PSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PSMD2 (IP:14748-1-AP, 4ug; Detection:14748-1-AP 1:1000) with K-562 cells lysate 3320ug.