OAAB05228

antibody from Aviva Systems Biology

Targeting: EHHADH ECHD

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB05228

Provider

Aviva Systems Biology

Product name

EHHADH antibody - C-terminal region

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of EHHADH Antibody (C-term) in mouse liver, kidney tissue lysates (35ug/lane). EHHADH (arrow) was detected using the purified Pab.

Sample type

mouse liver, kidney tissue lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Sample type

human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

HepG2 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol