GTX81126

antibody from GeneTex

Targeting: EHHADH ECHD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81126

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81126, RRID:AB_11163333

Product name

EHHADH antibody, C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Peroxisomes can oxidize medium- and long-chain fatty acids through a pathway involving ABCD3 and HSD17B4.

Violante S, Achetib N, van Roermund CWT, Hagen J, Dodatko T, Vaz FM, Waterham HR, Chen H, Baes M, Yu C, Argmann CA, Houten SM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Mar;33(3):4355-4364

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of EHHADH Antibody (C-term) (GTX81126) in mouse liver(lane 1), kidney(lane 2) tissue lysates (35ug/lane). EHHADH (arrow) was detected using the purified Pab.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term)(GTX81126), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term)(bottom histogram)(GTX81126) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.