Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-89747 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89747, RRID:AB_11024556
- Product name
- Rabbit Polyclonal GRASP55 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GRASP55 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Auto-regulation of Secretory Flux by Sensing and Responding to the Folded Cargo Protein Load in the Endoplasmic Reticulum.
Subramanian A, Capalbo A, Iyengar NR, Rizzo R, di Campli A, Di Martino R, Lo Monte M, Beccari AR, Yerudkar A, Del Vecchio C, Glielmo L, Turacchio G, Pirozzi M, Kim SG, Henklein P, Cancino J, Parashuraman S, Diviani D, Fanelli F, Sallese M, Luini A
Cell 2019 Mar 7;176(6):1461-1476.e23
Cell 2019 Mar 7;176(6):1461-1476.e23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRASP55 Antibody [NBP1-89747] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRASP55 Antibody [NBP1-89747] - Analysis in human cell line HepG2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GRASP55 Antibody [NBP1-89747] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.