12219-1-AP

antibody from Invitrogen Antibodies

Targeting: DYNC1I2 DIC74, DNCI2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12219-1-AP

Provider

Invitrogen Antibodies

Product name

DYNC1I2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HCQSAVMSA TFAKFHPNLV VGGTYSGQIV LWDNRSNKRT PVQRTPLSAA AHTHPVYCVN VVGTQNAHNL ISISTDGKIC SWSLDMLSHP QDSMELVHKQ SKAVAVTSMS FPVGDVNNFV VGSEEGSVYT ACRHGSKAGI SEMFEGHQGP ITGIHCHAAV GAVDFSHLFV TSSFDWTVKL WTTKNNKPLY SFEDNADYVY DVMWSPTHPA LFACVDGMGR LDLWNLNNDT EVPTASISVE GNPALNRVRW THSGREIAVG DSEGQIVIYD VGEQIAVPRN DEWARFGRTL AEINANRADA EEEAATRIPA (330-638 aa encoded by BC015038)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.18 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DYNC1I2 (IP:12219-1-AP, 4 ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug.