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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF2487 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Pappalysin-1/PAPP-A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Pappalysin-1/PAPP-A in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PAPP-A2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13219- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease.
Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Bolnick JM, Kohan-Ghadr HR, Fritz R, Bolnick AD, Kilburn BA, Diamond MP, Armant DR, Drewlo S
Scientific reports 2016 Sep 23;6:32382
Scientific reports 2016 Sep 23;6:32382
Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
The Journal of endocrinology 2009 Sep;202(3):337-45
The Journal of endocrinology 2009 Sep;202(3):337-45
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Nishizawa H, Pryor-Koishi K, Suzuki M, Kato T, Kogo H, Sekiya T, Kurahashi H, Udagawa Y
Molecular human reproduction 2008 Oct;14(10):595-602
Molecular human reproduction 2008 Oct;14(10):595-602
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Pappalysin-1/ PAPP-A by Western Blot. Western blot shows lysate of human pregnant sera. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Pappalysin-1/PAPP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pappalysin-1/PAPP-A at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
