Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- R31221 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Gelsolin Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids 763-775 (WDDDYWSVDPLDR-human) were used as the immunogen for this Gelsolin antibody (100% rat homology).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Gelsolin antibody and Lane 1: HeLa; 2: A431 cell lysate. Predicted/observed size ~86KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) (h) THP1, 3) (h) A549, 4) monkey COS-7, 5) (h) Caco-2, 6) (h) K562 and 7) (h) HL-60 lysate with Gelsolin antibody at 0.5ug/ml. Predicted molecular weight ~86 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of Gelsolin antibody and NIH3T3 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Gelsolin antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Gelsolin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Gelsolin antibody testing of rat heart tissue. HIER: steamed with pH6 citrate buffer.