NB110-55290

antibody from Novus Biologicals

Targeting: OPA1 FLJ12460, KIAA0567, MGM1, NPG, NTG

Provider product page for NB110-55290

Western blot

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [21]
Comments [0]
Validations
Western blot [4]
Immunohistochemistry [1]

Submit

Product number: NB110-55290 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB110-55290, RRID:AB_829789
Product name: Rabbit Polyclonal OPA1 Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified.
Reactivity: Human, Mouse, Rat, Chicken/Avian, Porcine, Zebrafish
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1 mg/ml
Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

OPA1 protein structure - NB110-55290 shown in red.

Submitted references Effects of deoxynivalenol on mitochondrial dynamics and autophagy in pig spleen lymphocytes.

Ren Z, Guo C, He H, Zuo Z, Hu Y, Yu S, Zhong Z, Liu H, Zhu L, Xu S, Deng Y, Hu H, Deng J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2020 Jun;140:111357

Hippo/Mst signaling coordinates cellular quiescence with terminal maturation in iNKT cell development and fate decisions.

Raynor JL, Liu C, Dhungana Y, Guy C, Chapman NM, Shi H, Neale G, Sesaki H, Chi H
The Journal of experimental medicine 2020 Jun 1;217(6)

Nitroxides affect neurological deficits and lesion size induced by a rat model of traumatic brain injury.

Zakarya R, Sapkota A, Chan YL, Shah J, Saad S, Bottle SE, Oliver BG, Gorrie CA, Chen H
Nitric oxide : biology and chemistry 2020 Apr 1;97:57-65

Mitochondrial transplantation regulates antitumour activity, chemoresistance and mitochondrial dynamics in breast cancer.

Chang JC, Chang HS, Wu YC, Cheng WL, Lin TT, Chang HJ, Kuo SJ, Chen ST, Liu CS
Journal of experimental & clinical cancer research : CR 2019 Jan 23;38(1):30

Burn-induced reductions in mitochondrial abundance and efficiency are more pronounced with small volumes of colloids in swine.

Chao T, Gómez BI, Heard TC, Smith BW, Dubick MA, Burmeister DM
American journal of physiology. Cell physiology 2019 Dec 1;317(6):C1229-C1238

Hippo/Mst signalling couples metabolic state and immune function of CD8α⁺ dendritic cells.

Du X, Wen J, Wang Y, Karmaus PWF, Khatamian A, Tan H, Li Y, Guy C, Nguyen TM, Dhungana Y, Neale G, Peng J, Yu J, Chi H
Nature 2018 Jun;558(7708):141-145

L-Carnitine and extendin-4 improve outcomes following moderate brain contusion injury.

Chen H, Chan YL, Linnane C, Mao Y, Anwer AG, Sapkota A, Annissa TF, Herok G, Vissel B, Oliver BG, Saad S, Gorrie CA
Scientific reports 2018 Jul 25;8(1):11201

Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers.

Chan YL, Saad S, Al-Odat I, Oliver BG, Pollock C, Jones NM, Chen H
Frontiers in molecular neuroscience 2017;10:33

Protective responses to sublytic complement in the retinal pigment epithelium.

Tan LX, Toops KA, Lakkaraju A
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 2;113(31):8789-94

Metalloprotease OMA1 Fine-tunes Mitochondrial Bioenergetic Function and Respiratory Supercomplex Stability.

Bohovych I, Fernandez MR, Rahn JJ, Stackley KD, Bestman JE, Anandhan A, Franco R, Claypool SM, Lewis RE, Chan SS, Khalimonchuk O
Scientific reports 2015 Sep 14;5:13989

Progressive dopaminergic alterations and mitochondrial abnormalities in LRRK2 G2019S knock-in mice.

Yue M, Hinkle KM, Davies P, Trushina E, Fiesel FC, Christenson TA, Schroeder AS, Zhang L, Bowles E, Behrouz B, Lincoln SJ, Beevers JE, Milnerwood AJ, Kurti A, McLean PJ, Fryer JD, Springer W, Dickson DW, Farrer MJ, Melrose HL
Neurobiology of disease 2015 Jun;78:172-95

High-lard and high-fish-oil diets differ in their effects on function and dynamic behaviour of rat hepatic mitochondria.

Lionetti L, Mollica MP, Donizzetti I, Gifuni G, Sica R, Pignalosa A, Cavaliere G, Gaita M, De Filippo C, Zorzano A, Putti R
PloS one 2014;9(3):e92753

Peroxiredoxin 3 has a crucial role in the contractile function of skeletal muscle by regulating mitochondrial homeostasis.

Lee KP, Shin YJ, Cho SC, Lee SM, Bahn YJ, Kim JY, Kwon ES, Jeong DY, Park SC, Rhee SG, Woo HA, Kwon KS
Free radical biology & medicine 2014 Dec;77:298-306

Opa1 is required for proper mitochondrial metabolism in early development.

Rahn JJ, Stackley KD, Chan SS
PloS one 2013;8(3):e59218

Mitochondrial fragmentation in cigarette smoke-induced bronchial epithelial cell senescence.

Hara H, Araya J, Ito S, Kobayashi K, Takasaka N, Yoshii Y, Wakui H, Kojima J, Shimizu K, Numata T, Kawaishi M, Kamiya N, Odaka M, Morikawa T, Kaneko Y, Nakayama K, Kuwano K
American journal of physiology. Lung cellular and molecular physiology 2013 Nov 15;305(10):L737-46

Mitochondria and tumor progression in ulcerative colitis.

Ussakli CH, Ebaee A, Binkley J, Brentnall TA, Emond MJ, Rabinovitch PS, Risques RA
Journal of the National Cancer Institute 2013 Aug 21;105(16):1239-48

Defects in mitochondrial dynamics and metabolomic signatures of evolving energetic stress in mouse models of familial Alzheimer's disease.

Trushina E, Nemutlu E, Zhang S, Christensen T, Camp J, Mesa J, Siddiqui A, Tamura Y, Sesaki H, Wengenack TM, Dzeja PP, Poduslo JF
PloS one 2012;7(2):e32737

Mitochondrial proteome remodelling in pressure overload-induced heart failure: the role of mitochondrial oxidative stress.

Dai DF, Hsieh EJ, Liu Y, Chen T, Beyer RP, Chin MT, MacCoss MJ, Rabinovitch PS
Cardiovascular research 2012 Jan 1;93(1):79-88

Thrombin-induced shedding of tumour endothelial marker 5 and exposure of its RGD motif are regulated by cell-surface protein disulfide-isomerase.

Vallon M, Aubele P, Janssen KP, Essler M
The Biochemical journal 2012 Feb 1;441(3):937-44

Regulation of acid-base transporters by reactive oxygen species following mitochondrial fragmentation.

Johnson D, Allman E, Nehrke K
American journal of physiology. Cell physiology 2012 Apr 1;302(7):C1045-54

High-mobility group box 1 is essential for mitochondrial quality control.

Tang D, Kang R, Livesey KM, Kroemer G, Billiar TR, Van Houten B, Zeh HJ 3rd, Lotze MT
Cell metabolism 2011 Jun 8;13(6):701-11

No comments: Submit comment

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OPA1 Antibody [NB110-55290] - Analysis of OPA1 in porcine retinal pigment epithelium lysate using anti-OPA1 antibody. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: OPA1 Antibody [NB110-55290] - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.