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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB9506-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human OPA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human, mouse, and rat OPA1 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
O60313- Isotype
- IgG
- Antibody clone number
- 1284B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat OPA1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PANC-1 human pancreatic carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human OPA1 Monoclonal Antibody (Catalog # MAB9506) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for OPA1 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
