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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-16149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OPA1 Monoclonal Antibody (1E8-1D9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- In Western blot, multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E8-1D9
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis.
Mechanical ventilation triggers abnormal mitochondrial dynamics and morphology in the diaphragm.
Mukherjee R, Chakrabarti O
Journal of cell science 2016 Feb 15;129(4):757-73
Journal of cell science 2016 Feb 15;129(4):757-73
Mechanical ventilation triggers abnormal mitochondrial dynamics and morphology in the diaphragm.
Picard M, Azuelos I, Jung B, Giordano C, Matecki S, Hussain S, White K, Li T, Liang F, Benedetti A, Gentil BJ, Burelle Y, Petrof BJ
Journal of applied physiology (Bethesda, Md. : 1985) 2015 May 1;118(9):1161-71
Journal of applied physiology (Bethesda, Md. : 1985) 2015 May 1;118(9):1161-71
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OPA1 in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates. Samples were incubated in OPA1 monoclonal antibody (Product # MA5-16149).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OPA1 in 1.0 mg/mL HeLa lysate. Samples were incubated in OPA1 monoclonal antibody (Product # MA5-16149). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OPA1 in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates. Samples were incubated in OPA1 monoclonal antibody (Product # MA5-16149).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OPA1 in 1.0 mg/mL HeLa lysate. Samples were incubated in OPA1 monoclonal antibody (Product # MA5-16149). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using OPA1 Monoclonal Antibody (1E8-1D9) (Product # MA5-16149) and 110, 107 kDa bands, corresponding to OPA1 was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1),T-47D (Lane 2), tissue extracts of Mouse brain (Lane 3) and Rat brain (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of OPA1 using a monoclonal antibody (Product # MA5-16149).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of OPA1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with OPA1 Monoclonal Antibody (Product # MA5-16149) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of OPA1 in mouse skin. Samples were incubated in OPA1 monoclonal antibody (Product # MA5-16149).
