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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- FAB2154G - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal B7-H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse B7-H4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7-H3, recombinant human (rh) B7-H3b, rhB7-H4, or rmPD-L2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of B7-H4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse B7-H4 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2154G) or (B) Rat IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC013G). View our protocol for Staining Membrane-associated Proteins.
